Primate cerebellum and primate nerves are the standard substrates for the determination of various neuronal antibodies. The parallel use of primate intestine permits the reliable differentiation from other autoantibodies (e.g. ANA) and makes it possible to distinguish between anti-Ri and anti-Hu.
Antibodies against Yo stain exclusively the cytoplasm of the Purkinje cells in the cerebellum. Clinical significance: paraneoplastic neurological syndromes (PNS), indication of a malignoma.
In the case of antibodies against Hu and Ri all neurone nuclei in the grey matter show a granular fluorescence. Hu antibodies react in the intestine with cell nuclei of the plexus myentericus, whereas Ri antibodies do not. Clinical significance: paraneoplastic neurological syndromes (PNS), indication of a malignoma.
