Detection of antibodies against islet cells of the pancreas (ICA).
Indication: Type I diabetes mellitus, differentiation between late manifestation of type 1 diabetes mellitus (latent autoimmune diabetes in adulthood, LADA) from type 2 diabetes.
For a reliable determination of antibodies against islet cells an extended incubation time of 18 hours for the patient serum must be observed. The incubation time may be reduced to 2 hours but this will lead to a decrease in the sensitivity of the antibody detection test.
Standardised control with JDF units available (order number CA 1021-0101-1).
With indirect immunofluorescence autoantibodies against pancreas islets (ICA) can be detected in 80% of patients with new-onset diabetes type 1. Characteristic target antigens of ICA are the enzyme glutamic acid decarboxylase (GAD) and tyrosine phosphatase (IA2).
This BIOCHIP can be supplemented by further substrates, e.g., primate cerebellum for detection of autoantibodies against GAD.
The use of small BIOCHIPs (1 x 1 mm) considerably facilitates the microscopic evaluation (order number FA 1020-1). The BIOCHIPS are virtually completely visible in the field of view, simplifying the location of the islet cells and, particularly for negative samples, eliminating the need for time-intensive examination.
